FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11792941
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAC33900.073.97571
CGAGTTG44700.071.896581
CTACTAG89050.065.737551
CGAGTAC28650.064.6861
CGTGTTG62550.064.665071
ATACTTG139050.063.9953961
ATACTAG108350.063.733981
TGACTAG102800.063.383321
GTAGTAG110700.063.311071
TCGTAGG25400.062.8290371
GCACTAG76400.062.760821
CGTAGGC27650.061.4522132
ACGTAGG32650.059.914621
CGTGTTC48500.059.4399831
TAGTAGG127350.058.8535232
TCACTAG102450.056.7804571
ACTAGGC105400.055.59723
TACTAGG107150.055.300412
CGTTGCA27100.054.9948122
CGAGTAA26500.054.9482231
Produced by FastQC (version 0.11.5)