FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20346768
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG92000.073.6032641
CGTGTAC58650.070.5769961
ATACTAG161600.067.5175861
CTACTAG146650.065.0082861
ATACTTG234050.062.947581
CGTGTTG107700.060.597151
CGAGTTG73150.059.4459571
GCACTAG131350.059.349211
CGAGTAC47850.058.7762641
GTAGTAG190800.057.865031
CGAGTAA43300.055.459461
TAAGTAG216200.053.83531
ACTAGGC180050.053.5655863
TCACTAG176350.053.0784871
CGTGTTC81650.052.8158571
TACTAGG172850.052.699532
CCGTTGC47950.052.487531
TCGTAGA107650.052.4772575
ACTAGGG180450.051.811533
TAGTAGG210000.051.4691472
Produced by FastQC (version 0.11.5)