FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9152073
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG35250.090.645411
CGTGTAC25000.085.3990251
CGTGTTG47200.078.3928761
CGAGTAA18750.078.09011
CGTAGGG16100.073.820962
ATACTAG82150.070.854461
CGTGTTC34600.070.25141
CGAGTTG33900.068.085011
ACGTAGC17000.066.611921
CGGTACA14450.065.8881451
TCGTAGG17850.065.864381
ATACTTG115600.065.576171
CCGTTGT17300.065.4567951
ACGTAGG23500.064.761341
CGGTACT12950.064.608471
CGTAGCC13550.064.3232042
CCGTTGC20950.064.036881
CGAGTAC21200.063.962171
CTACTAG72300.063.6478271
CGGTTGC14750.063.570051
Produced by FastQC (version 0.11.5)