FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9152073
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTG34650.073.252961
CGAGTAC21050.066.7987441
CGAGTAA18750.066.5321
GCACTAG59750.063.4795071
ATACTTG114700.063.181281
CGTGTTC31650.063.1090771
ATACTAG80200.063.02741
CGTGTAC30900.063.0073281
CCGTTGT19650.061.6499181
CGTGTTG53350.061.0927621
ACGTAGG23250.060.7881431
CGGTACA14900.060.4936071
CGGTACC13600.060.4437941
CGGTAGG17750.060.1241721
CTACTAG70400.059.6123281
CGTTGTC18300.059.0315932
TGACTAG89150.058.802911
CGGTAGC16250.057.686711
CGGTTGT18750.057.6867031
CGTAGCC15500.057.614832
Produced by FastQC (version 0.11.5)