FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21865842
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG84350.095.489741
CGTGTAC66200.081.8933261
CGTGTTG115600.077.1414261
CGAGTAA46300.072.8038561
CGTAGGG52850.072.2208252
ACGTAGG57700.072.097881
ATACTAG185850.071.795161
CGAGTAC46900.070.928841
CGAGTTG84650.069.969341
CGTGTTC85050.068.9464651
CTACTAG173950.067.1236651
GCACTAG140600.066.677521
GTAGTAG195750.066.3177951
TCGTAGG45650.065.7616651
ATACTTG267850.065.429551
CGGTATG25650.062.4011
CGGTACA30300.061.831571
TGACTAG184750.061.6028251
CGGTTGT42950.061.480731
CGGTAGG51000.061.1771621
Produced by FastQC (version 0.11.5)