FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21865842
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAC62250.071.429481
CGAGTAC47700.068.2569351
ATACTAG183200.065.873251
GTAGTAG199900.064.782031
ATACTTG266200.064.6606751
CGAGTTG82900.064.630041
CGTGTTG118300.064.114521
GCACTAG135200.064.0912251
CTACTAG166350.063.400191
ACGTAGG63450.062.665291
CGAGTAA46550.061.5752751
TGACTAG198200.061.4811941
CGTGTTC90550.061.3613361
CGTAGGG60400.058.6466372
TAGTAGG232950.056.49742
TCACTAG195750.056.0932041
ACGTAGC45500.056.0505031
TCGTAGG44900.055.1626321
TAAGTAG230450.054.950291
ACGTTGC56650.054.359511
Produced by FastQC (version 0.11.5)