FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4904750
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAA11450.066.7224351
CGTGTTG27900.064.064731
ATACTAG43400.063.2712941
ATACTTG60400.062.1687741
CGAGTAC10900.060.8321041
CGAGTTG19700.060.731411
CGTGTAC16150.060.2466241
CTACTAG37550.060.0766751
GCACTAG33300.059.5195921
CGTGTTC20400.059.0007861
GTAGTAG44450.058.3716851
ACGTACG3750.057.6582531
CGGTATG6950.057.0360451
CGAGTAT6550.056.1177641
TGACTAG45000.056.0566371
ACGTACC8350.055.2414361
CGTAGCA10700.055.1860662
TAAGTAG52300.054.5713841
CGGTACA8750.054.36351
TAGTAGG52700.054.247442
Produced by FastQC (version 0.11.5)