FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13041970
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACG6800.078.45641
ATACTAG110650.076.884031
CGTGTTC59250.074.8328551
CGTGTAC46300.073.963621
CGAGTAC35700.073.710651
ATACTTG161500.073.65751
CGTAGCC28900.072.766082
CCGTACG8250.072.532011
CTACTAG108050.069.52621
CGAGTTG48750.069.507041
ACGTAGG39500.067.714611
CGGTAGC29400.067.681081
CGTACGG8850.067.5427552
CGTAGGC41400.066.625792
GCACTAG91950.066.253761
ACGTTGC38200.065.678221
TCGTAGG27500.065.540971
GTAGTAG126000.065.114431
CGTACGC5350.064.6147162
CGTGTTG80350.063.4364851
Produced by FastQC (version 0.11.5)