FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11562225
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG48200.0103.032431
CGAGTTG43100.096.4706951
CGAGTAC21100.093.524081
CGTGTTG64900.088.63781
CGTGTAC31500.086.687661
CGTGTTC49600.085.590091
CGTAGGG21000.084.693132
CGAGTAA23150.084.613161
CGGTACG1900.084.3151551
CGTACGG3950.082.03252
TCGTAGG20850.078.929431
GCGTACG2400.075.851711
GCGTAGC13750.073.612011
GCGTACC9050.073.219951
GCGTAGG18800.072.8176351
ATACTTG161850.072.1202851
CGTAGGC20550.071.480712
CGTTGCA27100.071.209192
ATACTAG106800.069.68131
TCGTAGC14500.068.800111
Produced by FastQC (version 0.11.5)