FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11562225
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTG43450.095.255811
CGAGTAC22150.091.440281
CGTGTAC31500.088.0609741
CGTGTTG61400.083.66271
CGTACGG4400.080.3178562
CGTGTTC47250.077.8104861
CGAGTAA22450.075.181971
TCGTAGG22200.072.723021
CGTAGGG25650.071.138032
ATACTAG107600.070.110491
ATACTTG164050.070.0962751
GCGTACC9750.066.986541
TCGTACG3400.066.909071
GCACTAG78500.066.653361
TGACTAG120700.065.601891
GCGTTGC19750.065.023921
CGGTAGG18850.064.6247561
CTACTAG92250.064.593951
CGTAGGA22550.062.687112
CGTAGGC22300.062.4196242
Produced by FastQC (version 0.11.5)