FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21874642
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG94200.097.5952451
CGAGTTG86550.092.428691
CGTGTTG127050.086.1113741
CGTGTAC67650.084.357651
CGAGTAC47050.083.2336351
CGTGTTC96450.077.130271
TCGTAGG45950.076.355041
ATACTAG193500.075.7625661
ATACTTG300850.072.05281
CTACTAG175050.069.734411
CGTAGGC52000.068.126842
TGACTAG238050.067.944151
CGTAGGG52900.067.920572
CGAGTAA45000.067.291011
GCACTAG158700.066.965661
CGTAGGA45700.066.016782
GCGTAGG43350.064.814831
ACGTAGG52650.064.149871
GATCTAG183300.064.093991
TAAGTAG250450.061.9062841
Produced by FastQC (version 0.11.5)