FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21874642
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTG85300.093.883371
CGAGTAC49000.078.795721
CGTGTTG130750.076.406341
CGTGTAC66350.075.892031
ATACTAG196250.075.590911
ATACTTG304600.073.4992451
TGACTAG237750.071.195361
TCGTAGG44550.068.047991
CTACTAG174350.067.235081
CGAGTAA45750.067.065341
GCACTAG161000.066.7464451
CGTGTTC97500.064.8203661
GATCTAG182800.063.7255631
CGTAGGC51650.062.855972
CGTAGGG57800.062.6585352
TGACTTG388150.062.595241
ACGTAGG54300.062.0477181
GTAGTAG190600.061.1176951
TCGTTGC49750.060.1977121
TAAGTAG251800.059.9641231
Produced by FastQC (version 0.11.5)