FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12501745
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAC31900.0100.402731
CGTGTAG48700.091.9241641
CGTGTTC55200.089.539411
CGTTGCG2600.083.081272
GCGTAGG17750.082.839321
CGTAGGG19450.082.554452
GCGTACC8350.081.073691
GCGTTGC13300.079.359681
CGAGTAA24150.078.971011
CGTGTTG80200.077.511661
ATACTTG169800.075.7499541
TCGTAGG21650.074.977421
CGTAGGA24600.074.930752
GCGTAGC10100.074.2334061
GCGTACT8400.071.925411
GCGTACG3350.071.705531
GCACTAG73600.071.407341
CGTACCC13250.070.645212
CGTAGCC15500.070.145612
CGGTACG2700.070.095981
Produced by FastQC (version 0.11.5)