FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12501745
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAC31750.092.968451
CGTGTTC52700.085.687561
CGGTACG3150.081.58511
CGTACGG7600.074.990512
CGAGTAA25250.074.7351761
GCACTAG72500.074.439361
ATACTTG161200.073.9731751
CGTAGGA25850.073.3986362
CCGTACG5300.070.655771
TGACTAG122050.070.44861
GCGTACG4500.070.1631851
ATACTAG108250.070.001141
CGTAGGG23050.068.230482
CGTGTTG82650.068.0517351
GCGTAGC11750.067.489961
CTACTAG87900.067.412121
TCGTAGG24700.067.183831
GCGTACC8900.066.826441
CGGTTGC16750.065.316781
GCGTTGC15200.065.214391
Produced by FastQC (version 0.11.5)