FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16415067
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG74250.096.579861
CGAGTTG76450.089.2295761
CGTGTAC45500.088.3231351
ATACTTG215450.083.902911
CGTGTTG112000.083.137951
ATACTAG136400.082.249841
CGTACGG10350.082.0924152
CGAGTAC37150.079.1715851
TCGTAGG36750.079.04281
CGTGTTC79800.078.367691
CGTAGGG35000.075.0907062
TGACTAG169950.071.924411
CCGTACG7800.071.869211
CTACTAG128850.071.474791
GCACTAG117700.070.390261
GCGTAGG29550.070.215681
CGAGTAA29300.070.069381
CGTACGC6950.069.414692
GTAGTAG143500.069.2512361
TAAGTTG176600.068.7213441
Produced by FastQC (version 0.11.5)