FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18594100
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG80350.099.287061
CGAGTTG73300.092.451441
CGAGTAC41300.091.8238751
CGTGTTG112650.089.880181
CGTGTAC53700.089.3262561
ATACTTG254500.076.764911
TCGTAGG39950.076.5245061
ATACTAG162400.076.509531
CGTGTTC83900.076.172911
CGTAGGG41050.074.0227362
CGTACGG8400.073.719942
CGAGTAA36550.071.694051
CTACTAG150150.070.632121
CCGTACG7450.070.346781
GCACTAG134550.070.057411
GCGTAGG31650.068.5347061
GCGTACC16550.067.291691
TGACTAG196000.066.401741
CGTTGCA46650.065.445532
GTAGTAG166500.065.007691
Produced by FastQC (version 0.11.5)