FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3002992
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAC8650.093.40541
CGTGTTC13450.089.836111
CGTGTAG13300.089.754731
GCGTACT1850.086.559781
TCGTAGG4300.086.331021
ATACTTG36100.084.080251
GCGTACC2650.082.402531
CGGTATG2750.079.406071
CGTACGG1300.077.5486152
GCGTAGC2650.076.909031
CGTAGGG5650.076.4701842
CGTAGGA6650.075.799392
CGAGTAA5800.075.298851
GCACTAG17900.074.8221051
CGTGTTG18200.074.388651
CTACTAG19950.072.241611
CGTAGGT4300.072.009432
GCGTAGG5200.071.389111
ATACTAG25150.071.197091
TGACTAG26150.069.031151
Produced by FastQC (version 0.11.5)