FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9740467
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAC26600.095.2139741
CGTGTAG45000.087.657661
CGTGTTC44850.084.867681
CGTAGGA19100.083.689572
GCGTAGG13650.083.175421
TCGTAGG17200.082.933641
ATACTTG122400.082.0541841
CGTAGGG16100.080.053242
CGAGTAA17400.079.4707641
CGGTACG2950.078.946171
GCACTAG59850.075.392931
ATACTAG79650.074.834381
CTACTAG64100.074.82221
CGTAGCC15350.074.114032
TGACTAG89350.074.0410161
CGTGTTG71100.072.676141
TGTACGG22150.072.486333
GCGTTGC13850.071.990291
GCGTACG3250.071.658841
CGAGTAT13550.069.824391
Produced by FastQC (version 0.11.5)