FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21905903
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG100100.087.8721851
ATACTAG188950.081.312411
CGTGTTG131700.080.3993151
ATACTTG284700.076.4744951
CGAGTTG97250.076.342941
CGTGTAC71150.075.889361
CGAGTAA50300.071.564321
TGACTAG226450.070.1546251
CTACTAG171400.069.7797241
GCACTAG155800.068.894341
TCGTAGG53050.065.939321
GTAGTAG200350.065.746121
CGAGTAC53050.064.9817051
TAAGTAG232250.063.5279051
GATCTAG182250.062.8778151
CGTGTTC101150.062.7087861
TGACTTG363850.061.3545571
TCACTAG195300.061.2032051
TAAGTTG225700.060.3553241
CGTAGGG65650.058.0064542
Produced by FastQC (version 0.11.5)