FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21905903
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAG188400.078.1983261
ATACTTG287450.076.6269451
CGAGTTG94600.075.570691
TGACTAG215500.075.086741
CGTGTTG128200.071.811
CTACTAG176450.070.399541
CGTGTAC67400.069.8523251
TCGTAGG54500.065.387691
CGAGTAC56100.064.813911
GCACTAG147950.064.671841
TAAGTAG233100.063.824221
CGAGTAA51300.063.39521
GTAGTAG182450.062.6852871
CGTGTTC100300.060.7326741
GATCTAG175000.060.7182771
TGACTTG369800.060.601141
TCACTAG198900.059.904371
TAAGTTG224850.059.4978641
TACTAGG210150.058.5122682
GACTAGG221950.057.4773832
Produced by FastQC (version 0.11.5)