FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8911481
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG40000.092.6328351
CGAGTTG38950.082.421081
CGTGTAC26650.081.400341
ATACTAG76700.080.483651
ATACTTG120200.076.671931
CGAGTAA20300.074.947541
CGAGTAC21300.074.1629941
TGACTAG91150.074.11371
CGTGTTG54350.073.666551
CTACTAG71600.071.372561
GCACTAG64600.070.767571
TCGTAGG21300.070.403581
GTAGTAG77300.066.297991
CGTGTTC38800.064.915971
GATCTAG76950.064.42371
CGTAGGG23350.062.2885172
TGACTTG153400.060.6949231
TAAGTAG96750.060.4939881
TCACTAG85350.059.2772981
CGTAGGA23450.058.9524572
Produced by FastQC (version 0.11.5)