FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8911481
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTTG115300.075.3763051
CGAGTTG36250.074.921441
ATACTAG80500.073.9498751
TGACTAG89900.073.729361
CGTGTTG54450.066.729791
CGTGTAC26100.066.0906141
CGAGTAA20500.065.883461
CGAGTAC21300.065.476651
CTACTAG66250.064.926831
TAAGTAG92250.063.7350851
GATCTAG69750.063.036931
GTAGTAG77750.062.215341
TCGTAGG22300.060.2363551
GCACTAG63600.059.437781
GAACTAG78350.058.647271
TAAGTTG91300.058.368461
TCACTAG80850.058.0140651
TGACTTG142550.057.6717641
CGTAGGG29150.056.917342
ACGTAGG26800.056.421531
Produced by FastQC (version 0.11.5)