FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24495589
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG103950.098.10481
CGTGTAC69850.088.949971
CGAGTTG93750.086.635771
CGAGTAC57000.082.388741
CGTGTTG140150.081.724521
CGAGTAA51200.077.1200561
ATACTAG221050.075.588071
CGTAGGG50650.075.184782
TCGTAGG45750.073.932141
ATACTTG337600.073.6585851
CGTGTTC101500.071.3679051
TGACTAG251500.070.8519741
GCGTAGG42750.070.121691
CTACTAG201450.067.953661
CGTAGGA50400.067.130682
GCACTAG171400.066.443211
CGAGTAT32300.065.167931
GTAGTAG213900.064.541371
ACGTAGG55900.063.2347221
GATCTAG217350.063.0159841
Produced by FastQC (version 0.11.5)