FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17656298
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG74700.099.310891
CGTGTAC50950.090.0201341
CGAGTTG67000.089.535591
CGTGTTG105850.086.592081
CGAGTAC41250.081.361751
CGAGTAA38050.079.402961
CGTAGGG36300.074.1857452
ATACTAG161000.073.977681
TCGTAGG36750.073.693451
CGTGTTC71500.073.616561
ATACTTG246300.070.4077761
GCACTAG127150.069.509731
GCGTAGG29650.069.24171
TGACTAG182850.068.123721
CTACTAG154400.066.908051
CGTAGGA38900.066.080612
GTAGTAG156650.065.528771
CGAGTAT24350.064.878991
GCGTAGA26100.063.8760831
GCGTACT16250.063.169731
Produced by FastQC (version 0.11.5)