FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17656298
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAC40600.081.9353561
CGAGTTG67800.079.499711
CGAGTAA40300.075.8031851
CGTGTTG105350.073.957131
CGTGTAC48750.072.304581
ATACTTG243550.070.132731
ATACTAG163300.069.611981
TCGTAGG36400.068.9961
TGACTAG191950.068.212261
GCACTAG125550.065.3919451
CTACTAG149200.063.8366851
CGTAGGG42200.063.601372
CGTGTTC78200.062.822981
CGTAGGA40500.061.461072
GTAGTAG154700.059.525961
ACGTAGG44500.058.5824471
CGAGTAT24000.057.5235631
TGACTTG307950.057.4931641
TAAGTAG202200.057.490881
CGTAGGC38250.057.342712
Produced by FastQC (version 0.11.5)