FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18365024
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG76750.095.878171
CGAGTTG83400.091.200491
CGTGTAC55050.090.304691
CGTGTTG113150.086.6482851
CGTAGGG39800.079.420812
CGAGTAC44650.078.735871
ATACTTG247150.077.865991
CGTGTTC82900.077.614821
TCGTAGG39050.077.538831
ATACTAG158750.077.302011
GCGTAGG34100.073.426191
CGAGTAA37950.070.9638061
TGACTAG194300.070.538211
GCACTAG133700.070.444941
CGTTGCG6400.068.628222
CTACTAG154150.067.6155241
ACGTAGG44300.067.363881
CGTAGGA40850.066.627362
GTAGTAG165450.066.296931
CGTACGG9600.066.00312
Produced by FastQC (version 0.11.5)