FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22767580
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG99900.087.984071
CGAGTTG88150.078.056931
CGTGTTG125550.076.714881
ATACTAG177350.076.407941
ATACTTG282250.075.525811
CGTGTAC69150.073.2636641
CGAGTAC50450.071.07081
TCGTAGG53300.067.9514541
CGAGTAA49900.067.926891
CTACTAG164200.066.790811
TGACTAG225600.066.436451
CGTGTTC103650.063.443091
GTAGTAG183150.063.4071851
GCACTAG159250.062.622831
GATCTAG179500.061.7851831
TGACTTG370950.059.481731
TAAGTTG225000.058.774811
TAAGTAG234050.057.3704831
CGTAGGG67100.055.8957632
TCACTAG200000.055.7425271
Produced by FastQC (version 0.11.5)