FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7288156
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG30500.090.65391
CGTGTAC20850.078.51981
CGAGTTG27350.076.619251
ATACTAG64150.074.860031
ATACTTG91950.074.463011
CGTGTTG39550.074.325461
CGTGTTC31850.070.591391
CGAGTAC15350.070.154591
CGAGTAA16650.069.9211351
CTACTAG53600.067.60321
GCACTAG51250.065.3080441
TGACTAG70800.064.642791
TCGTAGG16700.064.047711
GATCTAG58800.063.2333641
GTAGTAG59250.062.5075041
TAAGTAG77550.062.300141
TAAGTTG72850.061.924841
TGACTTG120450.058.837591
CGTACGG5100.057.8826372
CGTAGGA16400.056.19542
Produced by FastQC (version 0.11.5)