FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11814989
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTG44300.088.972971
CGAGTAC24600.086.825131
CGTGTTG66850.081.248971
CGTGTAC29650.080.2061841
CGAGTAA25600.075.692271
CGTGTTC47500.071.3897551
TCGTAGG20650.069.310991
CGTAGGG22500.068.595692
ATACTTG169100.065.802621
TGACTAG114900.064.9663541
CGTAGGA22450.063.9296532
ATACTAG107550.063.468771
CGTAGAC16850.062.9191782
GCACTAG78900.062.886481
CGAGTTC32100.062.6517331
CGTACGG4500.062.505422
GCGTAGG18900.060.971291
CGAGTAT15900.060.9346541
GCGTACC9000.060.3499261
ACGTAGG24650.058.9570581
Produced by FastQC (version 0.11.5)