FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14026368
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAC27500.084.070191
CGAGTTG51300.083.5525361
CGTGTAC37900.080.366131
CGAGTAA28700.077.742031
CGTGTTG79050.073.719571
CGTGTTC59050.072.5867161
ATACTTG194200.069.237341
ATACTAG125550.068.045671
TGACTAG132700.065.596091
CGTAGGG28400.065.53142
TCGTAGG26500.063.4240231
GCACTAG90000.063.2824631
CTACTAG107100.061.3334961
ACGTAGG29000.059.7280271
CGTAGGA27350.057.233522
CGTACGA4600.056.4537472
TGACTTG247550.055.7982861
CGGTAGG25000.055.7799031
TAAGTTG155850.055.758241
GCGTAGG27000.055.453711
Produced by FastQC (version 0.11.5)