FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10111156
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG40350.097.2430651
CGAGTTG37150.096.409481
CGAGTAC19850.090.583651
CGTGTTG57050.086.6418151
CGTGTAC28500.085.823821
CGTGTTC41050.073.772341
CGAGTAA22450.073.283381
CGTAGGG18150.070.615672
ATACTAG93600.068.9082641
ATACTTG146200.068.216061
TCGTACG3900.067.197221
GCGTAGA15350.064.497661
GCGTAGG16650.064.271221
GCACTAG70850.064.1147841
CCGTTGT21000.063.7840231
TCGTAGG18350.063.4742161
TGACTAG104050.063.3169331
CGTACGC3300.063.276392
CGTACGA2850.063.161562
GATCTAG82700.062.498021
Produced by FastQC (version 0.11.5)