FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21073396
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG82350.096.252041
CGTGTAC59700.084.500891
CGAGTAC44350.083.796751
CGAGTTG77800.080.3293841
CGTGTTG117500.079.8751141
CGAGTAA44150.075.956011
ATACTAG177100.073.241431
TCGTAGG39900.072.039911
CGTGTTC84400.069.489591
CGTAGGG41850.068.803962
ATACTTG276300.067.331541
TGACTAG204050.064.279491
GCACTAG142750.063.6109731
CGAGTAT27700.061.8419381
CGTGTAA51250.061.0427741
CTACTAG162750.060.9675031
GATCTAG173900.059.270641
CGTAGGA41750.058.1061822
GCGTAGG36100.057.7066081
GCGTACA26800.057.418511
Produced by FastQC (version 0.11.5)