FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21073396
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTG81300.081.610541
CGTGTAC60900.078.975461
CGAGTAC44750.078.341821
CGAGTAA48650.075.634891
ATACTAG186950.072.491931
CGTGTTG114650.071.3281861
ATACTTG277500.067.422171
CGTGTTC90400.067.2256241
TGACTAG202900.067.186011
TCGTAGG43700.063.9804341
GCACTAG141800.061.246921
CGTAGGG47850.061.2358362
CTACTAG156200.059.4958881
CGTAGGA46050.058.0012632
GTAGTAG167700.057.6619761
GATCTAG163650.057.4513171
TAAGTAG225350.056.4749071
CGTTGCA54300.055.1552242
ACGTAGG50400.054.6127931
GGTGTAG164100.054.512951
Produced by FastQC (version 0.11.5)