FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27333744
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG115550.096.710171
CGAGTTG103900.094.502341
CGTGTTG152200.089.5741351
CGAGTAC53050.088.4419561
CGTGTAC74750.086.2442251
CGAGTAA56950.080.984671
CGTGTTC118600.076.49131
GCGTAGG41700.075.994971
CGTAGGG51900.075.922522
TCGTAGG53100.074.428861
GCGTACC20850.071.299631
CGTAGGA58650.069.763932
ATACTAG237850.069.178441
ATACTTG376350.068.769211
CGAGTAT37850.067.056611
TGACTAG274650.066.457351
GCACTAG191700.065.821321
CGTTGCA64650.064.514992
GCGTTGC41550.063.877731
CGTTGGG60500.063.463282
Produced by FastQC (version 0.11.5)