FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24269707
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG100750.096.3745351
CGAGTTG91950.093.249191
CGTGTTG136150.091.7383961
CGAGTAC48800.088.000211
CGTGTAC66450.085.766621
CGAGTAA53550.076.796331
CGTGTTC96550.075.387481
TCGTAGG46850.075.350071
CGTAGGG45900.074.984892
CGTAGGA50850.071.0839162
ATACTAG219450.070.050281
ATACTTG337050.069.212611
CTACTAG190800.068.590321
TGACTAG242650.067.882181
GCACTAG163100.067.743771
GCGTAGG38950.067.6476441
CGGTAGG35950.065.599061
ACGTAGG52050.063.766951
CGAGTAT32100.062.3561361
GCGTTGC34800.062.328771
Produced by FastQC (version 0.11.5)