FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10339058
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTG37900.086.191971
CGAGTAC22400.080.945921
CGAGTAA22200.074.400451
TCGTAGG20350.073.228081
ATACTTG136450.071.390991
CGTGTTG61500.070.6630861
ATACTAG90400.070.322691
TGACTAG102400.068.748731
CGTGTTC44450.067.545681
GCACTAG65500.066.571941
CGTGTAC32650.065.2019961
CTACTAG79750.061.8563961
TCGTAGC15000.061.6630941
GCGTACC9350.058.883781
CGTAGGA21050.058.6005672
CGTAGCC19800.058.2924922
TGACTTG172450.057.3816071
ACGTAGG22850.056.542171
CGAGTTA19300.056.2924921
CCGTACG5100.056.1358681
Produced by FastQC (version 0.11.5)