FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11334260
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTG43650.089.6352161
CGTGTAC33750.083.7380141
CGAGTAC24150.082.981491
TCGTAGG22550.079.428981
CGTGTTG67450.078.467361
ATACTAG107850.073.712991
CGAGTAA24300.073.40671
CGTGTTC45050.072.0216751
ATACTTG167700.069.2920761
TGACTAG120350.068.5576551
CGTAGGG25550.066.621012
GCACTAG77150.066.508471
CGTAGGC21650.064.629962
CTACTAG92300.062.7496071
CGTAGGT20200.062.485142
GCGTAGG22450.062.452921
TGACTTG198300.062.2272641
TCGTAGC16200.061.625381
CGTAGGA25900.061.5435562
GATCTAG92050.061.245351
Produced by FastQC (version 0.11.5)