FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15340152
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAC37950.083.9312441
CGAGTAA32800.077.195321
CGTGTTC64900.075.879351
ATACTTG197300.074.321441
TCGTAGG31300.072.922541
TGACTAG141450.072.275971
GCACTAG89400.070.682411
CGTAGGA31500.070.314862
ATACTAG136150.067.273291
CGAGTAT21700.066.6273041
CTACTAG111400.066.3422241
CGTAGGG28750.065.998972
CGTGTTG99050.064.5370941
ACGTAGG30800.063.621851
GCGTACC9700.062.7989041
GTAGTAG117000.062.539591
CGAGTTG90100.062.3135341
CGGTACA17400.061.581361
CGTAGCC16550.060.59492
CGTACTC16050.060.2350162
Produced by FastQC (version 0.11.5)