FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15501178
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAG142500.084.1900941
TGACTAG161850.078.205421
ATACTTG207600.075.549311
CTACTAG120600.074.2627261
CGTGTTG96600.071.45071
CGAGTTG73450.070.848461
CGTGTAC47300.069.969181
CGAGTAA37600.069.2962341
TCGTAGG40100.068.776851
GTAGTAG134000.068.623821
TAAGTAG169000.067.76781
GCACTAG113200.066.935591
CGTAGGG53250.066.269772
CGTAGGA40200.063.9559752
GATCTAG116800.063.8782961
CGAGTAC38600.063.3644521
TCACTAG147400.062.6314851
TAGTAGG167500.062.2576452
CGAGTAG119600.061.8973541
TAAGTTG170150.061.5939871
Produced by FastQC (version 0.11.5)