FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11134474
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG49950.098.241411
ATACTAG103850.084.269061
CGAGTTG53800.082.1441351
CGTGTTG66700.081.211461
TGACTAG114500.075.922231
CGAGTAC29500.075.768121
ATACTTG155150.074.894731
CTACTAG91900.074.787091
CGTGTAC35050.074.7798541
GTAGTAG99250.074.457021
GCACTAG84900.073.149521
CGAGTAA24950.069.450691
GATCTAG95500.068.68971
TCGTAGG27850.067.970161
TAAGTAG133900.066.9341351
CGTGTTC49900.066.82441
CGAGTAG89850.066.526591
CGTAGGG34950.065.3110352
CGTAGGA29600.063.9791532
GGTGTAG88450.063.957781
Produced by FastQC (version 0.11.5)