FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11134474
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAG103850.076.942651
TGACTAG114650.076.548841
CGAGTTG53600.074.812231
CTACTAG87300.073.694431
ATACTTG148650.072.676251
CGTGTAC35100.069.675791
GTAGTAG95400.068.900031
CGTGTTG69500.068.264121
GCACTAG77800.066.739751
TCGTAGG29550.065.861731
CGAGTAG79550.065.456211
CGAGTAC26350.064.105021
TAAGTAG126700.063.9357381
CGAGTAA26200.062.7901461
CGTAGGG36650.062.206682
GATCTAG85550.062.067331
TACTAGG113400.061.0776252
TAAGTTG123350.061.028051
TCACTAG102950.060.636951
TAGTAGG118350.059.3765032
Produced by FastQC (version 0.11.5)