FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9666812
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTG35450.094.83691
CGAGTAC18050.085.8091051
CGTGTAC25850.082.9183041
CGAGTAA22850.080.633521
CGTGTTG52750.080.015381
CGTAGGG20550.076.511262
CGTAGGA21550.075.972992
TCGTAGG19450.071.329691
GCACTAG65450.071.218391
CGTGTTC38150.069.460961
CGAGTAG60850.069.1229251
GCGTAGG16150.066.8148351
TGACTAG102400.066.165231
ACGTAGG22850.065.8560641
CTACTAG78050.065.74041
ATACTAG96550.064.928251
ATACTTG144550.064.797871
CGAGTTA19150.064.780761
CGCTAGG11750.064.346881
GTAGTAG91300.064.320191
Produced by FastQC (version 0.11.5)