FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5084870
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG22000.0106.894221
CGAGTTG18950.099.509581
CGTGTAC14700.095.095121
CGTAGCG850.093.178662
CGAGTAC10950.090.427211
CGTGTTG30200.088.477631
CGAGTAA12450.087.134731
CCGTACG1550.084.55031
TCGTAGG10550.082.813881
CGTACGA1150.081.393212
CGTAGGG10100.079.130572
GCGTAGG9050.077.231951
ATACTAG49250.075.3942641
CGTAGGA11750.074.146422
CGTGTTC20000.072.8072051
GCACTAG37350.070.565481
GCGTACC4550.070.406961
TGACTAG55350.070.373721
GATCTAG48500.069.6547241
ACGTAGG12200.068.6297451
Produced by FastQC (version 0.11.5)