FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5084870
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTG20800.093.930851
CGAGTAC10200.092.172151
CGTGTAC15150.081.449151
CGTGTTG29050.078.885721
TCGTAGG10100.075.6313551
CGAGTAA12000.074.6738361
CGTAGGC10500.073.523812
ATACTAG52050.072.391331
TGACTAG52900.070.117371
GCACTAG37150.068.8034741
CTACTAG43850.068.508371
CGTAGGG11150.067.2964252
CCGTACA7950.066.520471
CGGTAGG10500.065.754951
ATACTTG77350.065.520721
GCGTAGC7000.064.006151
GCGTAGG10350.063.869291
CGAGTAG38250.063.368351
GTAGTAG46700.063.069211
CGTGTTC21800.063.0051
Produced by FastQC (version 0.11.5)