FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19097585
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAC58650.079.179421
ATACTAG183500.078.570981
TGACTAG192200.074.297131
CGAGTAC45100.073.364951
CGAGTTG81750.073.3149641
ATACTTG253600.072.331621
CTACTAG152400.070.894091
CGTGTTG112550.069.949491
TCGTAGG50750.069.486521
GCACTAG135700.068.924461
GTAGTAG165250.066.74251
CGAGTAA44700.065.418091
GATCTAG145700.063.7954831
TAAGTAG218200.062.650781
TGACTTG325800.061.0684241
CGTAGGG61850.060.7805062
TACTAGG189000.060.4331052
TCACTAG182050.059.3876231
CGTGTTC89750.058.856881
ACGTAGG54700.058.6320761
Produced by FastQC (version 0.11.5)