FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8005934
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG37850.093.266031
CGAGTAC19050.082.911041
TCGTAGG18300.082.3316651
CGTGTAC25100.082.065441
CGAGTTG34450.080.920241
CGTGTTG48100.080.2007451
ATACTAG77300.078.058931
CTACTAG67850.075.843291
TGACTAG83900.074.000511
GTAGTAG70200.073.926481
GCACTAG58800.073.033521
ATACTTG110550.072.950561
CGTAGGG21750.068.1962
CGTAGGA21500.067.984292
CGAGTAA18000.067.933561
TAAGTAG91950.065.859621
CGTGTTC37000.065.3106461
GGTGTAG64700.064.123641
TCACTAG76050.063.071591
GATCTAG66050.062.2620241
Produced by FastQC (version 0.11.5)