FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18106272
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG75450.0101.613411
CGTGTAC47400.0100.457251
GCGTAGG22900.088.0696261
CGAGTAA40350.087.875391
CGTAGGG26750.087.214612
CGTGTTC66250.086.9305651
CGTAGGA32450.083.655542
TCGTAGG31200.081.676161
CGAGTAT24350.081.330191
GCGTAGC17050.080.281481
GCGTACC11150.079.664861
GCACTAG107000.077.911861
GCGTTGC22500.077.0151751
CGTGTTG113100.076.928481
GCGTACT13450.076.8684161
CGTACCG3250.075.329242
CGCTAGG16800.072.8084641
TGACTAG163450.072.028931
CGTAGCG3800.070.1112
CGGTACG3950.070.043591
Produced by FastQC (version 0.11.5)