FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18106272
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAC45000.088.760151
CGAGTAA37950.080.871621
GCACTAG108700.074.368561
CGTGTTC74500.073.225811
TGACTAG170600.073.035571
CGTAGGG36650.072.240352
TCGTAGG34700.071.306981
CGTAGGA36250.067.664152
CGTGTTG118750.067.518171
ATACTTG245550.067.188471
GCGTAGG33100.066.1028061
ATACTAG163650.065.952751
CTACTAG138900.065.388151
GTAGTAG142650.063.8236351
CGCTAGG18700.063.6070181
CGAGTAT26750.062.032071
CGAGTAG118400.061.3924671
CGAGTAC48750.060.6962781
GGTGTAG127950.060.540191
CGAGTTG95350.060.447811
Produced by FastQC (version 0.11.5)