FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18850723
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC312050.020.8504964
CGAGTAG51300.020.0120943
CCAAAGT367000.019.1349982
AAGTGCT352300.018.6639735
CCCAAAG443000.018.4160041
AGTAGCT199950.017.7774725
GCTGGGA581850.017.6964239
CCGAGTA61550.017.6058332
CAAAGTG385150.017.5266273
CTACTCG53950.017.3469892
TAGCTGG234700.016.4002177
GAGTAGC135700.016.258464
AGTGCTG431750.015.6182556
TACTCGG64800.014.7577763
GCTACTC161400.014.3609411
GTAGCTG242350.014.2308916
CTGTCTA144600.013.723458145
GGAGGCT438850.013.6679799
CCCGAGT99400.013.3957171
TTGGGAG341250.013.2146616
Produced by FastQC (version 0.11.5)