FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19068021
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC309900.019.7487664
CGAGTAG51350.019.5676633
CTACTCG53900.018.9027462
CCAAAGT371800.018.4831122
CCGAGTA56850.018.0454642
TACTCGG60600.017.6244013
AGTAGCT200650.017.5095185
AAGTGCT351650.017.464025
CCCAAAG455100.017.3924431
CAAAGTG387300.017.1264723
GCTACTC145100.017.1015831
GCTGGGA588850.016.8744329
TAGCTGG235750.016.5733727
GAGTAGC136550.014.97423654
GTAGCTG240600.014.6913376
AGTGCTG424300.014.27654656
GGAGGCT442050.013.4646919
TTGGGAG349000.012.7256686
CCCGAGT97300.012.7153671
GCTACTT143800.012.6121711
Produced by FastQC (version 0.11.5)